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Effects of isoform-specific and non-selective HDACIs on CLP-induced sepsis immune cell response. The spleens were homogenized into single-cell suspension and stained with anti-rat CD3, B220, <t>CD11b,</t> GR-1 and F4/80. Flow cytometry analysis was conducted on the different cell populations. Left representative of plots T lymphocytes, B lymphocytes, innate immune cells, macrophages and neutrophils are shown. Histograms on the right show relative changes in the indicated cell population. * P < 0.05, ** P < 0.01 and *** P < 0.001 for significant differences between the treatment and VEH groups. Sham: the peritoneal cavity was opened, but the cecum was eviscerated without ligated or punctured; VEH: vehicle; TubA: tubastatinA; TSA: trichostatin-A;
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Effects of isoform-specific and non-selective HDACIs on CLP-induced sepsis immune cell response. The spleens were homogenized into single-cell suspension and stained with anti-rat CD3, B220, CD11b, GR-1 and F4/80. Flow cytometry analysis was conducted on the different cell populations. Left representative of plots T lymphocytes, B lymphocytes, innate immune cells, macrophages and neutrophils are shown. Histograms on the right show relative changes in the indicated cell population. * P < 0.05, ** P < 0.01 and *** P < 0.001 for significant differences between the treatment and VEH groups. Sham: the peritoneal cavity was opened, but the cecum was eviscerated without ligated or punctured; VEH: vehicle; TubA: tubastatinA; TSA: trichostatin-A;

Journal: Surgery Open Science

Article Title: Protective effect of HDACIs in improves survival and organ injury after CLP-induced sepsis

doi: 10.1016/j.sopen.2023.03.003

Figure Lengend Snippet: Effects of isoform-specific and non-selective HDACIs on CLP-induced sepsis immune cell response. The spleens were homogenized into single-cell suspension and stained with anti-rat CD3, B220, CD11b, GR-1 and F4/80. Flow cytometry analysis was conducted on the different cell populations. Left representative of plots T lymphocytes, B lymphocytes, innate immune cells, macrophages and neutrophils are shown. Histograms on the right show relative changes in the indicated cell population. * P < 0.05, ** P < 0.01 and *** P < 0.001 for significant differences between the treatment and VEH groups. Sham: the peritoneal cavity was opened, but the cecum was eviscerated without ligated or punctured; VEH: vehicle; TubA: tubastatinA; TSA: trichostatin-A;

Article Snippet: The monoclonal antibodies were as follows: CD3 monoclonal antibody (1F4) (Thermo Fisher, product # MA5-16461), CD45R (B220) monoclonal antibody (HIS24) (eBioscience , product # 12-0460-82), F4/80 monoclonal antibody (BM8) (Santa Cruz, product # SC-377009), CD11b/c monoclonal antibody (OX42) (eBioscience, product # 12-0110-82) and anti-Ly-6G/Ly-6C (Gr-1) (RAT) fluorescein-conjugated monoclonal antibody (Rockland, product # 200-502-L54).

Techniques: Staining, Flow Cytometry